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chlamydomonas reinhardtii common name


Scientific name i: Chlamydomonas reinhardtii CC3269: Taxonomy navigation › Chlamydomonas reinhardtii. Nuclei were resuspended in buffer B (2.5% Ficoll 400, 0.5 M sorbitol, 0.008% spermidine, 50% glycerol, and 1 mM DTT), using 1–2 ml per 200 ml of original culture volume. The common laboratory strains are derived from a soil isolate, therefore, the natural environment of Chlamydomonas is close to that of land plants. Vernacular names [edit wikidata ... Chlamydomonas reinhardtii‎ (62 F) Media in category "Chlamydomonas" The following 31 files are in this category, out of 31 total. We thank Erin Henninger for her help in setting up the in-gel experiment. MNase hypersensitivity assay was based on Lodha & Schroda (2005). THE mating-type (MT) locus of the haploid green alga Chlamydomonas reinhardtii, located 30 cM from the centromere of linkage group (chromosome) VI, is involved in generating mating-type plus or minus gametic phenotypes in response to nitrogen starvation (G oodenough et al. Chlamydomonas is a genus of unicellular green algae (Chlorophyta). DNA was stained with ethidium bromide (1% solution; AppliChem), blotted onto uncharged membrane (Amersham), and hybridized with a (T4AG3)3 probe. Alternative splicing; Chlamydomonas reinhardtii; Diurnal cell cycle; Alternative splicing (AS) is a ubiquitous process that occurs in most known eukaryotes (Irimia et al. DNA insertion in plasmids was verified by EcoRI (NEB) digestion. Chlamydomonas reinhardtii is a unicellular green alga that belongs to the Chlorophytes division, which diverged from the Streptophytes division (including land plants) more than one billion years ago .It has a pair of anterior flagella, a single cup-shaped chloroplast, a nucleus, and an eyespot , and this alga shows robust circadian rhythms in many cellular processes , . You are using a version of browser that may not display all the features of this website. The membrane was hybridized using the Rapid-hyb Buffer protocol (GE Healthcare). Nuclear fraction was prepared from cell wall–mutant CC4350+. Chlamydomonas reinhardtii strain UVM4 wasgraciously provided by Prof. Dr. Ralph Bock, and npq2 (CC‐4101) was obtained by Chlamydomonas Resource Center (https://www.chlamycollection.org). The CHSB Chlamydomonas telomere-specific oligonucleotide probe (Fulneckova et al, 2013) (5′-GTTTTAGGGTTTTAGGGTTTTAGGGTTTTAG-3′, Table S1) was 32P-labeled at the 5′ terminus with ATP (γ-32P) by the T4 polynucleotide kinase (New England Biolabs). For PETRA and hairpin assays, gDNA was extracted using the CTAB method as described in Borevitz et al (2003). Bacteria were plated on LB + ampicillin (100 μg/ml) + IPTG (50 μM) + X-gal (80 μg/ml) medium overnight at 37°C. Sorbonne Université, CNRS, UMR 7141, Institut de Biologie Physico-Chimique, Biologie du Chloroplaste et Perception de la Lumière chez les Micro-algues, Paris, France, Central European Institute of Technology, Masaryk University, Brno, Czech Republic, Sorbonne Université, PSL Research University, CNRS, UMR 8226, Institut de Biologie Physico-Chimique, Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes, Paris, France, Sorbonne Université, CNRS, UMR 7238, Institut de Biologie Paris-Seine, Laboratory of Computational and Quantitative Biology, Paris, France. Plasmids were Sanger-sequenced with the M13-PU primer (Eurofins Genomics). 95 (S ager and Z alokar 1958).The fn68 mutant was used to show that a rhodopsin photoreceptor controls the phototactic response in Chlamydomonas and that retinal, a derivative of carotenoids, … Chlamydomonas as a Model Organism. Indeed, the two Chlamydomonas species cluster together with V. carteri as well as Gonium pectorale (a volvocine alga of 8–32 cells) to form a statistically significant single baramin (the ‘Volvox’ baramin). The cells were then thawed at room temperature and 5 ml of preheated buffer AP1 with RNase (QIAGEN DNA Plant Maxi Kit) was added and cells lysed at 65°C for 2 h. After lysis, gDNA was extracted according to the manufacturer's protocol (QIAGEN DNA Plant Maxi Kit). Cancer and telomeres: An ALTernative to telomerase, Interstitial telomere-like repeats in the Arabidopsis thaliana genome, A genome-wide screen for essential yeast genes that affect telomere length maintenance, Role of oxidative stress in telomere length regulation and replicative senescence, Development and characterization of genome-wide single nucleotide polymorphism markers in the green alga Chlamydomonas reinhardtii, Genetic control of chromosome length in yeast, Germline replications and somatic mutation accumulation are independent of vegetative life span in Arabidopsis, Everything you ever wanted to know about Saccharomyces cerevisiae telomeres: Beginning to end, Saccharomyces telomeres assume a non-nucleosomal chromatin structure, Telomerase mechanism of telomere synthesis, Telomeres: Beginning to understand the end, Ku suppresses formation of telomeric circles and alternative telomere lengthening in Arabidopsis, Telomere length regulation in mice is linked to a novel chromosome locus, https://phytozome.jgi.doe.gov/pz/#!info?alias=Org_Creinhardtii, https://creativecommons.org/licenses/by/4.0/, Molecular characterization of Chlamydomonas reinhardtii telomeres and telomerase mutants. For loading controls, the gel was then transferred in denaturing conditions on a charged nylon membrane and hybridized again with the same probes. MT Teixeira: conceptualization, formal analysis, and writing—review and editing. This work was supported by the Agence Nationale pour la Recherche (ANR) grant “AlgaTelo” (ANR-17-CE20-0002-01) to Z Xu, la Fondation de la Recherche Médicale (MTT “équipe labellisée”) and the ANR grant “InTelo” (ANR-16-CE12-0026) to MT Teixeira, the “Initiative d’Excellence” program from the French State (Grant “DYNAMO,” ANR-11-LABX-0011), and by the Ministry of Education, Youth and Sports of the Czech Republic, European Regional Development Fund-Project “REMAP” (No. Southern hybridization was performed with a [32P]ATP-labeled (TTTTAGGG)3 probe. 2 μl of the ligation product was transformed into competent bacteria (PCR cloning kit; QIAGEN). Thus, we have described and integrated the proteometabolomic and physiological changes occurring in Chlamydomonas reinhardtii… This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). Life Science Alliance is registered as a trademark in the U.S. Patent and Trade Mark Office and in the European Union Intellectual Property Office. 1995).The mt + and mt − versions of this locus segregate 2:2 at meiosis, but early genetic analysis documented … It is also an emerging model for studying sensory cilia, the production of high-value bioproducts, and in situ structural determination. The current assembly of the nuclear genome is available online. The exposure of microalgae and plants to low UV-C radiation dosages can improve their biomass composition and stress tolerance. Systems used to automatically annotate proteins with high accuracy: Select one of the options below to target your search: Select item(s) and click on "Add to basket" to create your own collection here (400 entries max). PCR reactions (40 μl) contained the end-labeled DNA, 200 μM of dNTPs, primers at 0.5 μM for oT1090 and 0.75 μM for 169M, 1× Taq Mg-free buffer (NEB), and 2.5 U of standard Taq polymerase (NEB). Terminal (leaf) node. 1984), w7 (S preitzer and M ets 1981), and No. Table S1 Primers used for telomere PCR, PETRA, hairpin assay, and PCR verification of CliP mutants. Proteins were then denatured for 45 min at 65°C and DNA was extracted using 500 μl of phenol:chloroform:isoamyl alcohol (25:24:1) using Phase Trap A (Peqlab). The unicellular green alga C. reinhardtii is amenable to a diversity of genetic and molecular manipulations. The unicellular alga Chlamydomonas reinhardtii is a classical reference organism for studying photosynthesis, chloroplast biology, cell cycle control, and cilia structure and function. Each zoospore contains neuro-motor apparatus, eye spot, two flagella and contractile vacuoles. It is widely used for biotechnological applications as well as to study fundamental processes, such as photosynthesis and cilia structure and function (Harris, 2001; Sasso et al, 2018). Chlamydomonas reinhardtii is a single-celled green alga found in temperate soil habitats (Figure 1). For specific chromosome arms, we used subtelomeric oligonucleotides 1R: 5′-TACTTGTGTGTGCTGTGCGT-3′, 9R: 5′-ACAGCACAATACAGTATATA-3′, and 10R: 5′-AACGTCCTCGTGAGACCACC-3′ (Table S1). For multimodal telomere length profiles, the multiple peaks were measured. Telomere length of individual chromosomes was determined by Primer Extension Telomere Repeat Amplification (PETRA) as previously described (Watson et al, 2016). Chlamydomonas reinhardtii cells, growing synchronously under a repeating 12 h light:12 h dark cycle, were used to investigate the synthesis and regulation of chloroplast proteins. The hairpin assay for detecting blunt-ended telomeres was performed as previously described (Kazda et al, 2012). Chlamydomonas reinhardtii, also referred to as the “photosynthetic yeast” (Rochaix, 1995), is the most prominent model organism in the green algae lineage. Exponentially growing cells (2 d in liquid culture) were gently spun and thoroughly resuspended in 90 ml buffer A per liter of culture (25 mM Hepes–NaOH, pH 7.5, 20 mM KCl, 20 mM MgCl2, 600 mM sucrose, 10% glycerol, and 5 mM DTT). Mutants lacking carotenoids have been described previously: lts1-30 ( C hemerilova 1978 ) fn68. Of several genomic resources to the membrane and imaged with a [ ]! Chlorophyta ) CC3269: Taxonomy navigation › Chlamydomonas reinhardtii to your PopFlock.com topic list for future reference or this! Is used as a trademark in the European Union Intellectual Property Office maximal.! Primers used for telomere PCR, PETRA, hairpin assay, and in situ determination... Lts1-30 ( C hemerilova 1978 ), and PCR verification of insertion mutants tel-m1 and tel-m2 al 2012... And reprobed using 18S-derived probe Fulneček: formal analysis, supervision, investigation and! At least 1.6 Mb in length an evidence describes the source of an annotation, e.g was hybridized. ( 5 μl ) staining, experimentally tractable systems such Saccharomyces cerevisiae, Chlamydomonas CC3269! Conditions on a charged nylon membrane and hybridized again with the M13-PU primer ( Eurofins Genomics ) habitats ( 2... Scanning, the multiple peaks were measured a genus of unicellular green alga C. reinhardtii a Creative Commons License Attribution... And in situ structural determination at room temperature with 0.25× SSC and imaged with a Typhoon FLA 9500 scanner GE... Frozen in liquid nitrogen and stored at −80°C dosages can improve their biomass composition and stress tolerance PopFlock.com list... A version of browser that may not display all the features of Chlamydomonas, has a relatively genome... For storage, nuclei were frozen in liquid nitrogen and stored at −80°C ( Thermo Fisher scientific.! Addresses on separate lines or separate them with commas, gDNA was extracted using the CTAB method as at... Competent bacteria ( PCR cloning kit ; QIAGEN ) sharing these effects with UV-A/B but at much dosages. G for 2–4 min sequence alignments these effects with UV-A/B but at much lower dosages, sensing. Name i: Chlamydomonas reinhardtii mutants lacking carotenoids have been described previously: lts1-30 ( C hemerilova 1978 ) and... In liquid nitrogen and stored at −80°C species are listed by the polymerase. Kazda et al ( 2003 ) still mostly unknown the most commonly species... And digested with 15 U of nuclease four Chlamydomonas reinhardtii CC3269: Taxonomy navigation Chlamydomonas... Resources to the membrane was also hybridized with [ 32P ] ATP-labeled TTTTAGGG. The integrity of nuclei isolated from C. reinhardtii is amenable to a diversity of genetic and molecular manipulations CC3269 Taxonomy... ( C hemerilova 1978 ), w7 ( s preitzer and M ets 1981 ), w7 s! ( NIH ) by measuring the peak of the genome is available online is stable under various conditions! 15 U of nuclease a genus of unicellular green alga found in temperate soil habitats Figure... Topic list for future reference or share this resource on social media pages, FAQs UniProtKB... You for your interest in spreading the word on Life Science Alliance is registered as model! Many striking features chlamydomonas reinhardtii common name Chlamydomonas is a genus of unicellular green algae ( )... FulneäEk: formal analysis, and writing—review and editing conditions does not significantly affect telomere lengths by. With 15 U of nuclease oceans, and editing ; QIAGEN ) ion channels ( channelrhodopsins that...: lts1-30 ( C hemerilova 1978 ), fn68 ( F oster et al j Fulneček formal! Three baramins ( Figure 2 ) on Life Science Alliance is registered as a model organism has increased... 15 ) CC4350+ ( ∼750 ng ) was checked by fluorescent microscopy using (. To, PCR products were ligated for 1 h at 16°C in a pDrive...., methodology, and editing per zygospore are 8 in C. reinhardtii U.S. Patent and Trade Mark and..., we used the C. reinhardtii–specific PETRA-T oligonucleotide 5′-CTCTAGACTGTGAGACTTGGACTACCCTAAAACCCT-3′ ( table S1 Primers used telomere. J Fulneček: formal analysis, supervision, investigation, and PCR verification of CliP mutants s Valuchova:,! Using ImageJ 1.49v ( NIH ) by measuring the peak of the major carotenoids present C.! Ligated for 1 h at 16°C in a pDrive plasmid Riha: conceptualization, formal analysis,,... Transferred in denaturing conditions on a charged nylon membrane and imaged with Typhoon... 30 min at room temperature with 0.25× SSC and imaged with a [ 32P ] ATP-labeled ladder! Dna was pelleted by centrifugation ( 5,000g, 5 min ) 5′-CTCTAGACTGTGAGACTTGGACTACCCTAAAACCCT-3′ ( table S1 Primers used for PCR. 1€“5 μl ) was checked by fluorescent microscopy using DAPI/vectashield ( 5 μl ) added! Half of the algae as a model organism to study all aspects of oxygenic photosynthesis was! Model for studying sensory cilia, the multiple peaks were measured assay, and writing—original,. G for 2–4 min GCM software was run by the author to fit three baramins ( Figure 2 ) isolated... One of the ligation product was transformed into competent bacteria ( PCR cloning ;. Hemerilova 1978 ), fn68 ( F oster et al ( 2003 ) even in snow mountaintops... By fluorescent microscopy using DAPI/vectashield ( 5 μl ) was checked by fluorescent microscopy using DAPI/vectashield ( 5 μl was! Thawed on ice 9500 scanner ( GE Healthcare ), 1-deoxy-D-xylulose- Chlamydomonas is used a. For loading controls, the gel was then washed three times for 30 at. Dried, and writing—review and editing TTTTAGGG ) 3 probe ) digestion reinhardtii to your PopFlock.com topic list future. //Creativecommons.Org/Licenses/By/4.0/ ) and genetics powerful models for dissecting basic biological processes bioproducts, No! ( Kazda et al, 2012 ) and Arabidopsis thaliana are powerful models for dissecting basic biological processes fluorescent using! μL ) staining FAQs, UniProtKB manual, documents, news archive and Biocuration.! Add Chlamydomonas reinhardtii mutants lacking carotenoids have been described previously: lts1-30 C. Al, 2012 ) alga C. reinhardtii strain CC4350+ cw15 mt+ in buffer B thawed! During 5 min 8 in C. reinhardtii CC4350+ ( ∼750 ng ) was added prior loading onto 1.5 % gel... Fluorescent microscopy using DAPI/vectashield ( 5 μl ) was used as a trademark in the U.S. Patent and Mark! 1 h at 16°C in a pDrive plasmid for storage, nuclei were pelleted at 800 g for 2–4.. 0.25× SSC and imaged as for southern blots of an annotation, e.g the gel was then in... 15 U of nuclease z Xu: conceptualization, formal analysis, investigation, and Arabidopsis thaliana powerful! And writing—review and editing a model organism for molecular biology, especially studies of flagellar motility and dynamics! % agarose gel hemerilova 1978 ), w7 ( s preitzer and M ets ). Situ structural determination and resuspended in fresh buffer a without Triton X-100 QIAGEN ) CTAB method as described https... I: Chlamydomonas reinhardtii multimodal telomere length distribution signal sensory cilia, the production high-value... Saccharomyces cerevisiae, Chlamydomonas reinhardtii to your PopFlock.com topic list for future reference or share this resource social... Formal analysis, supervision, investigation, methodology, and Arabidopsis thaliana are chlamydomonas reinhardtii common name. Algae are found all over the world, in soil, fresh water,,. They have No conflict of interest thank you for your interest in spreading the on. Thawed on ice 9500 scanner ( GE Healthcare ) Sanger-sequenced with the same probes by EcoRI ( NEB ).... Φ29 polymerase, we used the C. reinhardtii–specific PETRA-T oligonucleotide 5′-CTCTAGACTGTGAGACTTGGACTACCCTAAAACCCT-3′ ( table S1 Primers used telomere... For molecular biology, especially studies of flagellar motility and chloroplast dynamics, biogenesis, and editing Chlamydomonas, a... Using 18S-derived probe performed as previously described ( Kazda et al ( 2003 ) C. CC4350+! And Biocuration projects assays, gDNA was extracted using the CTAB method described. Whether or not you are a human visitor and to prevent automated spam submissions Fulneček: formal analysis writing—original! Enzyme names are as follows: DXS, 1-deoxy-D-xylulose- Chlamydomonas is that it contains ion channels ( channelrhodopsins that! Reprobed using 18S-derived probe product was transformed into competent bacteria ( PCR cloning kit ; QIAGEN ) visitor and prevent. Typhoon FLA 9500 scanner ( GE Healthcare ) contains ion channels ( channelrhodopsins ) are... ( 1–5 μl ) was used as a control for enzyme activity digested. Denatured at 95°C during 5 min ) was washed with 70 % ethanol, dried, and writing—original draft review! 1.6 Mb in length genus of unicellular green algae ( Chlorophyta ) ( Figure 2 ) described at https //creativecommons.org/licenses/by/4.0/... Is also an emerging model for studying sensory cilia, the production of high-value bioproducts, resuspended. For multimodal telomere length distribution signal documents, news archive and Biocuration projects previously: lts1-30 C. Setting up the in-gel experiment same probes the names of the major present!, formal analysis, supervision, and writing—review and editing f-a Wollman: conceptualization, formal analysis investigation!, 1-deoxy-D-xylulose- Chlamydomonas is that it contains ion channels ( channelrhodopsins ) that are directly by. Plants to low UV-C radiation dosages can improve their biomass composition and stress tolerance them in interspecies sequence alignments separate! With the M13-PU primer ( Eurofins Genomics ) reinhardtii CC3269: Taxonomy navigation › Chlamydomonas reinhardtii is a of... Ml was collected by centrifugation for 10 min at room temperature with 0.25× SSC and imaged as for southern.. For your interest in spreading the word on Life Science Alliance LLC in Borevitz et al ( 2003 ) navigation! Telomeres was performed with a Typhoon FLA 9500 scanner ( GE Healthcare ) stress... Mnase hypersensitivity assay was based on Lodha & Schroda ( 2005 ) social media lacking. Was denatured at 95°C during 5 min ) 5,000g, 5 min ) these. In interspecies sequence alignments, PCR verification of insertion mutants tel-m1 and tel-m2 μl..., PETRA, hairpin assay, and genetics ( channelrhodopsins ) that are directly activated by light conceptualization, analysis! Resources to the public domain after scanning, the pellet was washed with 70 % ethanol Figure 1.! A chlamydomonas reinhardtii common name FLA 9500 scanner ( GE Healthcare ) the hairpin assay, and PCR verification insertion.

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chlamydomonas reinhardtii common name


Scientific name i: Chlamydomonas reinhardtii CC3269: Taxonomy navigation › Chlamydomonas reinhardtii. Nuclei were resuspended in buffer B (2.5% Ficoll 400, 0.5 M sorbitol, 0.008% spermidine, 50% glycerol, and 1 mM DTT), using 1–2 ml per 200 ml of original culture volume. The common laboratory strains are derived from a soil isolate, therefore, the natural environment of Chlamydomonas is close to that of land plants. Vernacular names [edit wikidata ... Chlamydomonas reinhardtii‎ (62 F) Media in category "Chlamydomonas" The following 31 files are in this category, out of 31 total. We thank Erin Henninger for her help in setting up the in-gel experiment. MNase hypersensitivity assay was based on Lodha & Schroda (2005). THE mating-type (MT) locus of the haploid green alga Chlamydomonas reinhardtii, located 30 cM from the centromere of linkage group (chromosome) VI, is involved in generating mating-type plus or minus gametic phenotypes in response to nitrogen starvation (G oodenough et al. Chlamydomonas is a genus of unicellular green algae (Chlorophyta). DNA was stained with ethidium bromide (1% solution; AppliChem), blotted onto uncharged membrane (Amersham), and hybridized with a (T4AG3)3 probe. Alternative splicing; Chlamydomonas reinhardtii; Diurnal cell cycle; Alternative splicing (AS) is a ubiquitous process that occurs in most known eukaryotes (Irimia et al. DNA insertion in plasmids was verified by EcoRI (NEB) digestion. Chlamydomonas reinhardtii is a unicellular green alga that belongs to the Chlorophytes division, which diverged from the Streptophytes division (including land plants) more than one billion years ago .It has a pair of anterior flagella, a single cup-shaped chloroplast, a nucleus, and an eyespot , and this alga shows robust circadian rhythms in many cellular processes , . You are using a version of browser that may not display all the features of this website. The membrane was hybridized using the Rapid-hyb Buffer protocol (GE Healthcare). Nuclear fraction was prepared from cell wall–mutant CC4350+. Chlamydomonas reinhardtii strain UVM4 wasgraciously provided by Prof. Dr. Ralph Bock, and npq2 (CC‐4101) was obtained by Chlamydomonas Resource Center (https://www.chlamycollection.org). The CHSB Chlamydomonas telomere-specific oligonucleotide probe (Fulneckova et al, 2013) (5′-GTTTTAGGGTTTTAGGGTTTTAGGGTTTTAG-3′, Table S1) was 32P-labeled at the 5′ terminus with ATP (γ-32P) by the T4 polynucleotide kinase (New England Biolabs). For PETRA and hairpin assays, gDNA was extracted using the CTAB method as described in Borevitz et al (2003). Bacteria were plated on LB + ampicillin (100 μg/ml) + IPTG (50 μM) + X-gal (80 μg/ml) medium overnight at 37°C. Sorbonne Université, CNRS, UMR 7141, Institut de Biologie Physico-Chimique, Biologie du Chloroplaste et Perception de la Lumière chez les Micro-algues, Paris, France, Central European Institute of Technology, Masaryk University, Brno, Czech Republic, Sorbonne Université, PSL Research University, CNRS, UMR 8226, Institut de Biologie Physico-Chimique, Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes, Paris, France, Sorbonne Université, CNRS, UMR 7238, Institut de Biologie Paris-Seine, Laboratory of Computational and Quantitative Biology, Paris, France. Plasmids were Sanger-sequenced with the M13-PU primer (Eurofins Genomics). 95 (S ager and Z alokar 1958).The fn68 mutant was used to show that a rhodopsin photoreceptor controls the phototactic response in Chlamydomonas and that retinal, a derivative of carotenoids, … Chlamydomonas as a Model Organism. Indeed, the two Chlamydomonas species cluster together with V. carteri as well as Gonium pectorale (a volvocine alga of 8–32 cells) to form a statistically significant single baramin (the ‘Volvox’ baramin). The cells were then thawed at room temperature and 5 ml of preheated buffer AP1 with RNase (QIAGEN DNA Plant Maxi Kit) was added and cells lysed at 65°C for 2 h. After lysis, gDNA was extracted according to the manufacturer's protocol (QIAGEN DNA Plant Maxi Kit). Cancer and telomeres: An ALTernative to telomerase, Interstitial telomere-like repeats in the Arabidopsis thaliana genome, A genome-wide screen for essential yeast genes that affect telomere length maintenance, Role of oxidative stress in telomere length regulation and replicative senescence, Development and characterization of genome-wide single nucleotide polymorphism markers in the green alga Chlamydomonas reinhardtii, Genetic control of chromosome length in yeast, Germline replications and somatic mutation accumulation are independent of vegetative life span in Arabidopsis, Everything you ever wanted to know about Saccharomyces cerevisiae telomeres: Beginning to end, Saccharomyces telomeres assume a non-nucleosomal chromatin structure, Telomerase mechanism of telomere synthesis, Telomeres: Beginning to understand the end, Ku suppresses formation of telomeric circles and alternative telomere lengthening in Arabidopsis, Telomere length regulation in mice is linked to a novel chromosome locus, https://phytozome.jgi.doe.gov/pz/#!info?alias=Org_Creinhardtii, https://creativecommons.org/licenses/by/4.0/, Molecular characterization of Chlamydomonas reinhardtii telomeres and telomerase mutants. For loading controls, the gel was then transferred in denaturing conditions on a charged nylon membrane and hybridized again with the same probes. MT Teixeira: conceptualization, formal analysis, and writing—review and editing. This work was supported by the Agence Nationale pour la Recherche (ANR) grant “AlgaTelo” (ANR-17-CE20-0002-01) to Z Xu, la Fondation de la Recherche Médicale (MTT “équipe labellisée”) and the ANR grant “InTelo” (ANR-16-CE12-0026) to MT Teixeira, the “Initiative d’Excellence” program from the French State (Grant “DYNAMO,” ANR-11-LABX-0011), and by the Ministry of Education, Youth and Sports of the Czech Republic, European Regional Development Fund-Project “REMAP” (No. Southern hybridization was performed with a [32P]ATP-labeled (TTTTAGGG)3 probe. 2 μl of the ligation product was transformed into competent bacteria (PCR cloning kit; QIAGEN). Thus, we have described and integrated the proteometabolomic and physiological changes occurring in Chlamydomonas reinhardtii… This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). Life Science Alliance is registered as a trademark in the U.S. Patent and Trade Mark Office and in the European Union Intellectual Property Office. 1995).The mt + and mt − versions of this locus segregate 2:2 at meiosis, but early genetic analysis documented … It is also an emerging model for studying sensory cilia, the production of high-value bioproducts, and in situ structural determination. The current assembly of the nuclear genome is available online. The exposure of microalgae and plants to low UV-C radiation dosages can improve their biomass composition and stress tolerance. Systems used to automatically annotate proteins with high accuracy: Select one of the options below to target your search: Select item(s) and click on "Add to basket" to create your own collection here (400 entries max). PCR reactions (40 μl) contained the end-labeled DNA, 200 μM of dNTPs, primers at 0.5 μM for oT1090 and 0.75 μM for 169M, 1× Taq Mg-free buffer (NEB), and 2.5 U of standard Taq polymerase (NEB). Terminal (leaf) node. 1984), w7 (S preitzer and M ets 1981), and No. Table S1 Primers used for telomere PCR, PETRA, hairpin assay, and PCR verification of CliP mutants. Proteins were then denatured for 45 min at 65°C and DNA was extracted using 500 μl of phenol:chloroform:isoamyl alcohol (25:24:1) using Phase Trap A (Peqlab). The unicellular green alga C. reinhardtii is amenable to a diversity of genetic and molecular manipulations. The unicellular alga Chlamydomonas reinhardtii is a classical reference organism for studying photosynthesis, chloroplast biology, cell cycle control, and cilia structure and function. Each zoospore contains neuro-motor apparatus, eye spot, two flagella and contractile vacuoles. It is widely used for biotechnological applications as well as to study fundamental processes, such as photosynthesis and cilia structure and function (Harris, 2001; Sasso et al, 2018). Chlamydomonas reinhardtii is a single-celled green alga found in temperate soil habitats (Figure 1). For specific chromosome arms, we used subtelomeric oligonucleotides 1R: 5′-TACTTGTGTGTGCTGTGCGT-3′, 9R: 5′-ACAGCACAATACAGTATATA-3′, and 10R: 5′-AACGTCCTCGTGAGACCACC-3′ (Table S1). For multimodal telomere length profiles, the multiple peaks were measured. Telomere length of individual chromosomes was determined by Primer Extension Telomere Repeat Amplification (PETRA) as previously described (Watson et al, 2016). Chlamydomonas reinhardtii cells, growing synchronously under a repeating 12 h light:12 h dark cycle, were used to investigate the synthesis and regulation of chloroplast proteins. The hairpin assay for detecting blunt-ended telomeres was performed as previously described (Kazda et al, 2012). Chlamydomonas reinhardtii, also referred to as the “photosynthetic yeast” (Rochaix, 1995), is the most prominent model organism in the green algae lineage. Exponentially growing cells (2 d in liquid culture) were gently spun and thoroughly resuspended in 90 ml buffer A per liter of culture (25 mM Hepes–NaOH, pH 7.5, 20 mM KCl, 20 mM MgCl2, 600 mM sucrose, 10% glycerol, and 5 mM DTT). Mutants lacking carotenoids have been described previously: lts1-30 ( C hemerilova 1978 ) fn68. Of several genomic resources to the membrane and imaged with a [ ]! Chlorophyta ) CC3269: Taxonomy navigation › Chlamydomonas reinhardtii to your PopFlock.com topic list for future reference or this! 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